HelixCript™ Easy cDNA Synthesis Kit은 기존 HelixCript™ First cDNA Synthesis Kit의 Pre-mix 타입으로 역전사(Reverse Transcriptase)를 위한 반응요소 혼합물을 손 쉽게 준비할 수 있습니다. 본 제품의 RT Enzyme Mix에는 Helixcript™ Thermo Reverse Transcriptase와 RNase Inhibitor가 혼합되어 있고, 5X RT Reaction Mix에는 dNTP mix와 DTT가 혼합되어 있습니다. 이런 혼합형의 제품은 시간절약뿐만 아니라 여러 반응요소들의 혼합과정에서 발생하는 error와 오염률을 최소화 시켜 줍니다. HelixCript™ Easy cDNA Synthesis Kit을 통해 빠르고 간편하게 First-strand cDNA 합성 반응을 진행할 수 있습니다.

 

Figure 1. RT-PCR using HelixCript™ 1st-Strand cDNA Synthesis Kit and random hexamer or oligo-dT as primers for reverse transcription. 1st-strand cDNA synthesis reactions were performed using  300 ng of total RNA isolated from human blood. 10% of each 1st-strand cDNA product was used in PCR reactions with primer sets corresponding to fragments of human dynein gene and 1.25 units HelixAmp™ Xtender-Taq Polymerase in 50 ul of PCR reaction. 

Application 

• Generation of the first-strand cDNA for subsequent PCR or Real-time PCR 
• cDNA library construction 

Contents 

• 50 pmole/㎕ oligo d(T20) primer
• 50 pmole/㎕ random hexamers 
• 5x RT Reaction Mix
• RT Enzyme Mix
• DEPC water 

Quality Control

HelixCript™ Easy cDNA Synthesis Kit was functionally tested for RT-PCR amplification on different products size (0.5 ~ 5 kb) with 500 ng of total RNA.